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. Author manuscript; available in PMC: 2015 Sep 7.
Published in final edited form as: J Biomol Screen. 2014 Mar 7;19(7):1060–1069. doi: 10.1177/1087057114526433

Figure 5. Counter-screen with DNA transcription factor SP1 to assess specificity of identified hit compounds.

Figure 5

(A) Overview of TR-FRET assay for measurement of SP-1 binding to DNA. (B) Transcription factor SP-1 binding to methylated and unmethylated oligonucleotides in the TR FRET assay. There appears to be no dependence of the methylation status on protein-DNA binding. (C) All 4 compounds showing a dose-responsive inhibition of MBD2-DNA binding were also capable of inhibiting SP1-DNA binding in a dose-dependent manner.