FIGURE 2.

Evidence for blood group Lewis-independent carbohydrate specificity of TFF2 (hTFF2-Fl) binding to mucins. A, triplicate assay (with S.D.) of TFF2 binding to blood group Lewis-defined human gastric mucin (HGM, 5 μg/well) and to a blood group undefined sample (5 μg/well) tested prior to and after mild periodate oxidation of carbohydrates (10 mm sodium metaperiodate in 0.1 m sodium acetate, pH 4.0, overnight incubation at 4 °C in the dark). Average background binding was at 0.06 (A_{405 nm}). B, triplicate assay (with S.D.) of TFF2 binding to neoglycoproteins (0.5 μg/well) carrying blood group-related carbohydrate antigens. Average background binding was at 0.06 (A_{405 nm}).