FIGURE 3.

TFF2 (hTFF2-Fl)-binding competition with GSA II and other lectins. A, upper panel, triplicate assays (with S.D.) of TFF2 binding to immobilized PSM glycopeptides (0.5 μg/well) and inhibition assays using lectin competitors (500 μg/ml); lower panel, duplicate binding inhibition assays with 5-fold dilution series of G. simplicifolia agglutinin isolectin II. Average background binding was at 0.06 (A_{405 nm}). B, triplicate assay (with S.D.) of TFF2 (expressed in HEK-293 cells) binding to PSM glycopeptides (0.5 μg/well) and inhibition by human TFF2 expressed in E. coli (10-fold molar excess) in the presence (1 mm) or absence of calcium ions. Average background binding was at 0.06 (A_{405 nm}).