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Gastric mucin-derived hexasaccharides inhibit TFF2 (hTFF2-Fl) binding. A, duplicate ELISA inhibition assay of hTFF2-Fl binding to PSM-glycopeptides (0.5 μg/well) by dried aliquots from polar-phase HPLC-separated PSM-derived glycan alditols (fractions 5–19, estimated concentration range of glycan alditols applied: 50–500 μm). Average background binding was at 0.06 (A405 nm). B, representative chromatogram of neutral glycan alditols (250 μg) from PSM separated by polar-phase HPLC.
