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H,H-TOCSY NMR spectrum of oligosaccharides in fraction PSM-N10 (expansion), obtained at 600 MHz (303 K, 80 ms spin lock). Spin system assigned for terminal GlcNAcα1-4. The hexasaccharide analyte (approximately 100 μg) dissolved in water was repeatedly dried by vacuum rotation and resolubilization in deuterated solvent (D2O). The final concentration was 0.2 mm.
