FIGURE 1.

GX sPLA₂ is expressed in pancreatic islet cells and MIN6 cells. A, total RNA was isolated from mouse pancreas, islets isolated from mouse pancreas, and MIN6 cells, a mouse pancreatic beta-cell line. RT-PCR was performed using 0.5 μg of RNA and primers for GX sPLA₂ and 18 S. B, pancreatic sections from WT and GX KO mice were stained by indirect immunofluorescence for GX sPLA₂ (green) and insulin (red) and visualized (20× magnification) by fluorescence microscopy. A merged image shows co-expression of GX sPLA₂ in insulin-producing cells of WT pancreas (yellow).