FIGURE 2.

Smc5 binds to microtubules via electrostatic interaction with its hinge domain and adjacent coiled-coil arm. A, domain structure of Smc5 constructs. FL, full length; HM, Hinge domain with medium coiled-coil extensions; HL, Hinge domain with long coiled-coil extensions. B, microtubule co-sedimentation with truncation constructs of Smc5. A representative Coomassie Blue-stained gel of supernatant (S) and pellet (P) fractions is shown (n = 3). C, quantification of the percentage of Smc5 constructs associated with microtubules. Averages of three independent experiments are shown. Error bars, S.D. D, co-sedimentation assay with full-length Smc5 using untreated paclitaxel-stabilized microtubules (MT) and subtilisin-treated microtubules (sMT).