FIGURE 8.

Reducing Smc5 binding to microtubules in vivo induces a mitotic arrest. A, cell cycle progression of yeast carrying SMC5, SMC5-STH, tub2-401, and smc5-3KE after a G₁ arrest using α-factor. After release at 18 °C, samples of the cell cultures were taken every 30 min to evaluate the budding index and DNA content. Small budded cells were scored when the bud was smaller than one-third of the mother cell, whereas big budded cells were scored when the bud was bigger than one-third of the mother cell. B, flow cytometry analysis showing the DNA content of the yeast strains used in the time course experiment.