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Native gel electrophoresis of CCT5 and its neuropathy mutant. M. marapaludis chaperonin (Mm-Cpn, control), WT CCT5, and H147R CCT5 were subjected to native gel electrophoresis. Vertical lines are for visual comparison and indicate the chaperonin complexes in each lane. The H147R CCT5 mutant ran slightly slower than WT CCT5, suggesting that the mutation alters the outer charge of the mutant chaperonin.
