FIGURE 7.

Far-UV CD scans and thermal denaturation of CCT5 and its neuropathy mutant. A, CD scans of WT CCT5 (blue) and H147R CCT5 (magenta) showed similar spectra from 260 to 195 nm, with minima at ∼227 nm. B, thermal denaturation of WT CCT5 (blue) and H147R CCT5 (magenta) by CD had approximately the same midpoint of 60 °C, although the profiles were slightly different in terms of cooperativity. The mean molar ellipticity at 227 was used as the proxy for the percentage of protein folding. The WT CCT5 scan and thermal melt are consistent with those published by Sergeeva et al. (14).