FIGURE 3.

Decreased expression of genes involved in phosphate homeostasis in Trps1-OE cells. A, qRT-PCR graphs depicting Phex, Vdr, and Fam20C mRNA expression during osteo-odontogenic differentiation in Trps1-OE cells in comparison with Cntr cells. Data are presented as the mean relative levels of Phex, Vdr, and Fam20C mRNA (normalized to β-actin) ±S.D. from three stable cell lines. Day 0 values for each cell line were arbitrarily set to 1 and used as a reference for remainder of days of differentiation. B, top panel, Western blots of Phex, Vdr, and Fam20C expression on day 6 and Phex expression on day 8 of differentiation on protein extracts isolated from Trps1-OE and Cntr cell lines. Bottom panel, results of densitometric quantification of the Western blot images. Protein levels were normalized to tubulin or Gapdh as depicted in the top panel. Asterisks denote statistically significant differences (*, p ≤ 0.05; **, p ≤ 0.005).