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SDS-PAGE analysis of γ′-Fg and γA-Fg. To confirm their purity, γ′-Fg and γA-Fg were subjected to SDS-PAGE analysis under reducing conditions. Prior to electrophoresis, additional aliquots of Fg were incubated with 10 nm thrombin and 10 mm CaCl2, and the absence of γ-γ dimers was used as evidence that the preparations were devoid of factor XIII. Locations of the α, β, γ′ and γA chains are indicated on the left, and the molecular weights of the markers are indicated on the right (MW).
