FIGURE 6.

Influence of FXIIIa on tPA-mediated lysis of γ′-Fn or γ_A-Fn clots. γ′-Fg (triangles) or γ_A-Fg (circles), at concentrations ranging from 4 to 16 μm, was incubated at 37 °C with 0.33 μm α₂-antiplasmin, 0.67 μm Pg, and 5 mm CaCl₂ in HBS containing 0.01% Tween 80, in the presence (open symbols) or absence (closed symbols) of 20 nm FXIIIa. Reactions were initiated by adding the mixtures to wells of a 96-well plate containing 5 nm thrombin and 0.25 nm tPA, and absorbance was monitored at 400 nm. Lysis times were calculated as the time to half-maximal decrease in absorbance. Symbols represent the mean of at least three experiments, while the bars above the symbols reflect S.D. Lines were generated by linear regression analysis.