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N-Glycan Structures of Nxph1 and Nrxn1α
Results are from nanoESI quadrupole time of flight mass spectrometry. Glycans of a complex type were found at Asn-146 and Asn-162 of Nxph1 and at Asn-125, Asn-190 and Asn-797 of Nrxn1α. The high mannose type N-glycan exclusively attached to Asn-156 was shown by analyzing wild-type and mutant proteolytic glycopeptides where Asn-162 is inactivated by mutation to Asp. A = Gal, AN = GalNAc, GN = GlcNAc, F = Fuc, M = Man, NA = NeuAc.
