TABLE 1.
Yeast strains
| Strain | Genotype | Source |
|---|---|---|
| GT81-1C | MATa ade1-14 (UGA), his3-Δ200, leu2-3,112 lys2-801, trp1-289 ura3-52 [PSI+ PIN+] | 62 |
| GT982 | get2Δ::HISMX6 derivative of GT81-C | 31 |
| GT1929-1C | kanMX6::PGAL-GFP-SBH1 in GT81-C | 31 |
| MHY501 | MATα his3- Δ200 leu2-3,112 lys2-801, trp1-1 ura3-52 | 63 |
| MHY3646 | doa3-1 derivative of MHY501 | 63 |
| WTY623 | lsb2Δ::kanMX6 disruption of MHY501 | 26 |
| WTY659 | lsb1Δ::HIS3MX6 disruption of MHY501 | This study |
| WTY660 | lsb2Δ::kanMX6, lsb1Δ::HIS3MX6 in MHY501 | This study |
| pJ69-4A | PGAL-ADE2 [psi−] | 37 |
| FW1808 | MATα, rsp5-1, his4-912δR5, lys2-128δ, ura3-52 | 42 |
| FY56 | MATα, his4-912δR5, lys2-128δ, ura3-52 | 42 |
| OT55 | MATa, ade1-14 (UGA), his3-Δ200 leu2-3, 112 ura3-52, trp1-289 [PSI+] [PIN+] | 64 |
| WTY682 | lsb2Δ::kanMX6 disruption of OT55 | 26 |
| WTY730 | lsb1Δ::HIS3MX6 disruption of OT55 | This study |
| WTY731 | lsb1Δ::HIS3MX6 disruption of WTY682 | This study |
| WTY637 | lsb1Δ::KlURA3, hyg, GAL1-I-SceI disruption of WTY 682 | This study |
| WTY643 | lsb1 Y182A, Y183A replacement of WTY 637 | This study |
| WTY649 | lsb1 K79R replacement of WTY 637 | This study |
| WGC4a | MATa leu2-3, 112 ura3, his3-11, 15CanS, GAL2 | 44 |
| YUS5 | pup1-T30A, pre3-T20A mutant of WGC4a | 44 |
| ACY1568 | MATα MLP1-RFP-kanMX6 leu2, his3, ura3,lys2 | 47 |