TABLE 2.

Viability of dissected mother and daughter cells in yeast cultures treated with mild heat shock

Exponential yeast cultures were incubated at 39 °C for the specified periods of time. Mother and daughter cells were separated from each other by micromanipulation. Numbers are shown for each type of mother/daughter.

	Time at 39 °C
	30 min	60 min	240 min
WT
Viable mother and daughter	265 (64%)	427 (68%)	75 (69%)
Viable mother	5 (1%)	9 (1%)	0 (0%)
Viable daughter	17 (4%)	12 (2%)	3 (3%)
Delay in budding	21	26	3
Petitte	1	5	4
Total viable	309 (75%)	479 (77%)	85 (79%)
Total cells analyzed	412	624	108
lsb1Δ lsb2Δ
Viable mother and daughter	318 (52%)	371 (49%)	207 (41%)
Viable mother	18 (3%)	24 (3%)	13 (3%)
Viable daughter	23 (4%)	26 (3%)	36 (7%)
Delay in budding	32	36	29
Petitte	15	14	6
Total viable	406 (66%)	471 (62%)	296 (59%)
Total cells analyzed	612	757	499