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. 2014 Aug 6;289(40):27665–27676. doi: 10.1074/jbc.M114.570341

FIGURE 3.

FIGURE 3.

MED14 and MED27 impaired HIV-1 replication in MDM. A, mRNA knockdown efficiency in MDM cells was quantified by qPCR 72 h post-transfection. mRNA expression of each MED gene was normalized to a sample treated without siRNA (Mock). Means ± S.D. of three independent experiments are shown. B, MDM were transfected with the indicated siRNA and infected (INF) 72 h later with vesicular stomatitis virus-pseudotyped NL4-3-GFP virus. Infection was measured as the percentage of GFP-positive cells in siRNA-treated macrophages and expressed as the percentage to mock-treated cells. AZT was used as a control (white bars). In parallel, cell viability was assayed in uninfected (UN) siRNA-treated macrophages by counting live cells using flow cytometry (black bars).Means ± S.D. of three independent donors are shown. RALT, raltegravir. *, p < 0.05.