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Effect of siRNA-mediated NRAS gene suppression on phosphorylation state of MAPKs and AKT in vemurafenib-resistant A375rVem and DM443rVem cells. Cells were transfected with 10 nm stealth NRAS-targeted or scrambled (control) siRNA for 48 h and analyzed for total and phosphorylated proteins by Western blotting, as described under “Experimental Procedures.”
