TABLE 4.

Changes in total and activated MAPKs and RAS-associated proteins following acquisition of vemurafenib resistance in A375 and DM443 melanoma cells

Western blot bands were quantified by densitometry and used to compute the phosphoprotein/protein or the protein/GAPDH ratios. Each value is the mean of triplicate determinations ± S.D.

	A375	A375rVem	Fold change	DM443	DM443rVem	Fold change
Phosphoprotein
JNK/phospho-JNK	0.31 ± 0.026	1.46 ± 0.50	4.71	0.42 ± 0.02	1.53 ± 0.05	3.64
ERK/phospho-ERK	0.81 ± 0.075	4.13 ± 0.13	5.10	0.87 ± 0.03	4.25 ± 0.15	4.88
p38/phospho-p38	0.86 ± 0.02	1.26 ± 0.06	1.46	0.14 ± 0.012	0.83 ± 0.04	5.93
MEK/phospho-MEK	0.45 ± 0.01	1.13 ± 0.15	2.5	0.25 ± 0.02	0.93 ± 0.02	3.74
ARAF/phospho-ARAF	0.065 ± 0.005	0.02 ± 0.005	0.03	0.063 ± 0.005	0.06 ± 0.005	0.95
CRAF/phospho-CRAF	1.35 ± 0.022	1.24 ± 0.03	0.92	0.11 ± 0.02	0.44 ± 0.03	4.0
AKT/phospho-AKT	0.13 ± 0.02	1.96 ± 0.16	15.08	0.22 ± 0.016	1.16 ± 0.03	5.27
Total protein
BRAF/GAPDH	0.92 ± 0.87	1.2 ± 0.24	1.30	0.75 ± 0.05	1.32 ± 0.05	1.76
NRAS/GAPDH	0.053 ± 0.01	3.21 ± 0.05	6.01	0.076 ± 0.01	1.56 ± 0.13	1.56
GSTP1/GAPDH	1.36 ± 0.035	0.74 ± 0.03	0.54	1.73 ± 0.025	1.15 ± 0.03	1.15