FIGURE 3.

ILK inhibitor QLT-0267 inhibits LPS-induced NF-κB-Luc activation and LPS-induced TNF-α transcription in RAW264.7 cells. A, RAW264.7 cells were transfected with plasmids containing NF-κB-Luc reporter (40 ng) and Renilla-Luc (5 ng). 18 h after transfection, cells were left untreated or treated with QLT-0267 (1, 2, 5, 10, and 20 μm) or vehicle (DMSO) for 1 h, followed by LPS treatment for 6 h. B, RAW264.7 cells were pretreated with QLT-0267 (5, 10 μm) followed by LPS treatment for 2 h. mRNAs were extracted, and TNF-α and GAPDH mRNA expressions were measured by real time PCR as described under “Experimental Procedures.” TNF-α mRNA expression was normalized against GAPDH. Data are representative of three different experiments. p values were calculated by two-tailed t test and were relative to LPS stimulation alone: *, 0.01 < p ≤ 0.05; ***, p ≤ 0.001; NT, not treated.