TABLE 2.
Primers used in this study
F, forward primer; R, reverse primer.
| Primer ID | Sequence (5′ to 3′) | Application |
|---|---|---|
| DB-96-F | tataggatccatgaaggatgctaatgagag | PRO1-GFP genotyping |
| DB-102-F | gcgaatatgtcgctcatagagaaaatttggcattcccacctcgtcggatccccgggttaattaa | GFP tagging for PRO1 |
| DB-103-R | taagaaatatcattaagggaatcctctataactttttagttcgttgaattcgagctcgtttaaac | GFP tagging for PRO1, PRO1 knock out |
| DB-116-F | ggtcattttatcagctactgtaatatagtcataagaacgcgaaacggatccccgggttaattaa | PRO1 knock out |
| DB-174-F | ttgagcaccagttaaagtatgaaca | PRO1 knockout genotyping |
| DB-98-F | tataggatccatgtccagttcacaacaaat | PRO2 CDNA synthesis, PRO2-GFP genotyping |
| DB-99-R | tcaactcgagtcaagcgtaatctggaacatcgtatgggtataatgtcacagtctttatat | PRO2 cDNA synthesis |
| DB-120-F | caaagcttttgttcacaaggatttagatataaagactgtgacattacggatccccgggttaattaa | GFP tagging for PRO2 |
| DB-121-R | gtatacacgtccttcatacatggaacttagctttatcctaaaaatttgaattcgagctcgtttaaac | GFP tagging for PRO2 |
| DB-100-F | tataggatccatgacttacacattggcaatt | PRO3 cDNA synthesis, PRO3-GFP genotyping |
| DB-101-R | tcaactcgagtcaagcgtaatctggaacatcgtatgggtatttcttcttttggcctaatt | PRO3 cDNA synthesis |
| DB-122-F | gaagaggcagcccgtgttgcgtcacaattaggccaaaagaagaaacggatccccgggttaattaa | GFP tagging for PRO3 |
| DB-105-R | tatacggctacaaagcgaacatacatacatttcacgcaaagcaatgaattcgagctcgtttaaac | GFP tagging for PRO3, PRO3 knock out |
| DB-104-F | aatagaagtaacacaacaccaatagcaaacaaaccaactacagatcggatccccgggttaattaa | PRO3 knock out |
| DB-175-F | ataaagtcgcatacgtacaaaagga | PRO3 knockout genotyping |
| DB-173-R | gtcgattacagcaaatggtc | GFP fusion mutant and gene knockout mutant genotyping |
| DB-158-F | agaaggcctccaagggtatt | KAR2 mRNA levels |
| DB-159-R | taagacaccagcttgaacgg | KAR2 mRNA levels |
| DB-176-F | caaggcatgatgcagatttc | SLT2 mRNA levels |
| DB-177-R | ctagaagcgtgccgttatca | SLT2 mRNA levels |
| DB-182-F | ccttaaacgaaggcgaagag | GSC2 mRNA levels |
| DB-183-R | gggacgtctacgaccattct | GSC2 mRNA levels |
| DB-156-F | ccaagatgttcgttggaatg | GSH1 mRNA levels |
| DB-157-R | cctccaaatccgttcttgtt | GSH1 mRNA levels |
| DB-140-F | atttggccggtagagatttg | ACT1 mRNA levels |
| DB-141-R | tccaaggcgacgtaacatag | ACT1 mRNA levels |
| DB-223-F | ttggcagacccactctg | HAC1i mRNA levels |
| DB-225-R | ctgcgcttctggattacg | HAC1i mRNA levels |