FIGURE 7.
Polymorphisms determine ROP5 inhibition of IRGa6. A, the predicted structural consequences of mutating ROP5CI (black) at polymorphic residues that interact with IRG (orange) helix 3 are shown shaded. In particular, mutation of Gly-490 is predicted to clash with Asn-163 in IRG helix 3 and with the short helical stretch at IRG residues 129–132. B, oligomerization of IRGa6 was monitored after the addition of GTP in the presence or absence of wild-type or mutant ROP5CI. C, IRGa6 GTPase activity was measured, as in Fig. 4, in the presence 150 μm wild-type or mutant ROP5CI. Error bars, S.D.