FIGURE 5.

Hsp83 is required for JH-induced Kr-h1 expression through JHRR. A and B, the JH-induced Kr-h1 expression (A) and JHRR-driven luciferase activity (B) were measured after Kc cells were pretreated with 1 μg/ml of GA (DMSO as the control) for 2 h or after Hsp83 was reduced by RNAi (EGFP RNAi as the control). C, the mRNA levels of Kr-h1 in the fat body isolated from w¹¹¹⁸, Hsp83⁰⁸⁴⁴⁵ (an Hsp83 weak mutant), and two Hsp83 RNAi (32996 and 33947; Lsp2-GAL4>UAS-Hsp83-RNAi) larvae at 3 h after the initiation of wandering when the JH titer is high. D, the JHRR-driven β-gal activities were measured in the fat body isolated from the JHRR-LacZ::Lsp2-GAL4/+ and JHRR-LacZ::Lsp2-GAL4/UAS-Hsp83-RNAi larvae at 3 h AIW. EGFP, enhanced green fluorescent protein.