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. 2014 Aug 13;289(40):27874–27885. doi: 10.1074/jbc.M114.582825

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Hsp83 facilitates JH-induced Met nuclear import. Immunohistochemistry with the anti-V5 antibody reveals the subcellular localization of Met-V5 in fat body cells isolated from the indicated genotypes and different treatments. GA, in vitro treatment with 1 μg/ml of geldanamycin for 2 h; JHA, in vitro treatment with 1 μm methoprene for 2 h; GA+JHA, GA for 2 h and JHA for 2 h; DMSO, the control treatment. A, Met-V5 was detected at 3 h AIW when the JH titer is high. B, heat shock was performed at 6 h AEL for 15 min, and Met-V5 was detected in clones of Met-V5-overexpressed fat body cells, which were marked with GFP, at 3 h AIW. C, Met-V5 was detected at 96 h AEL when the JH titer is low. D, heat shock was performed at 6 h AEL for 15 min, and Met-V5 was detected in cell clones at 96 h AEL.