Hsp83 is accumulated in the cytoplasm area adjunct to the nucleus in the presence of JH and Met/Gce. Immunohistochemistry with the anti-Hsp83 antibody reveals the subcellular localization of Hsp83 in fat body cells isolated from the indicated genotypes (A and A′, the w1118 larvae; B and B′, the Lsp2-GAL4>UAS-Met-V5 larvae; C and C′, the Met27gce2.5k larvae) at 96 h after egg laying, without (left panels, DMSO as control) or with (right panels) in vitro treatment with 1 μm methoprene (JHA) for 2 h.