FIGURE 2.
Purification of recombinant rHCLase from E. coli by Ni2+ chelation chromatography. Enzyme purity following each fractionation step was assessed by SDS-PAGE using 13.2% polyacrylamide gels followed by staining with Coomassie Brilliant Blue. Lane 1, unstained protein molecular weight marker SM 0431 (Thermo); lane 2, uninduced cell lysate; lane 3, induced cell lysate; lane 4, supernatant fluid of the induced cell lysate; lane 5, purified recombinant rHCLase. Molecular weight markers and their corresponding masses are also indicated.