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. 2014 Aug 13;289(40):27886–27898. doi: 10.1074/jbc.M114.590752

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Time course experiments of rHCLase degradation of HA and CS-A. HA (A) or CS-A (B) (1 mg/ml) was treated with rHCLase (1 unit/ml), and a 10-μl aliquot was taken at different time points for gel filtration analysis as described under “Experimental Procedures.” The elution positions of the following standard oligosaccharides are indicated by arrows: 1, CS or HA octasaccharide fraction; 2, CS or HA hexasaccharide fraction; 3, CS or HA tetrasaccharide fraction; 4, disulfated CS disaccharide fraction; 5, monosulfated CS disaccharide fraction; 6, nonsulfated CS or HA disaccharide.