FIGURE 5.

Analysis of the final products of HA and CS digested by rHCLase. Ten micrograms each of HA (A), CS-A (B), CS-C (C), CS-D (D), or CS-E (E) was exhaustively digested with rHCLase and then separated into its constituent oligosaccharides by gel filtration chromatography on a Superdex Peptide column as described under “Experimental Procedures.” The elution positions of the following standard oligosaccharides are indicated by arrows: 1, CS tetrasaccharides; 2, CS disulfated disaccharides; 3, CS monosulfated disaccharides; 4, nonsulfated CS or HA disaccharides.