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. 2014 Aug 13;289(40):27886–27898. doi: 10.1074/jbc.M114.590752

FIGURE 8.

FIGURE 8.

Analysis of the final products of 2-AB-labeled oligosaccharides digested by rHCLase. Five picomoles of 2-AB-labeled HA tetrasaccharides (A) or hexasaccharides (B) was exhaustively digested without (top panel) or with rHCLase (100 units/ml) (bottom panel) at 30 °C for 72 h. The resulting product was analyzed by gel filtration as described above. 1, 2-AB-labeled unsaturated HA hexasaccharide; 2, 2-AB-labeled unsaturated HA tetrasaccharide.