FIGURE 5.

Binding of bacitracin to BceB. SPR spectroscopy was used to quantify interactions between BceB and Zn²⁺-bacitracin or nisin. BceB-Strep was captured onto a Strep-Tactin® XT-coated chip before increasing concentrations of Zn²⁺-bacitracin or nisin (control) were injected. A, single cycle binding kinetic of bacitracin binding to BceB. The gray line shows the recorded sensorgram, and the black line shows the fitted sensorgram. B, steady-state affinity of bacitracin (black) and nisin (gray) to BceB. RU, response units.