TABLE 1.
Plasmids and strains used in this study
| Plasmid or strain | Descriptiona | Ref. or source |
|---|---|---|
| Plasmids | ||
| pASK-IBA5 plus | Vector for tetracycline-inducible gene expression; carries an N-terminal Strep-tag II® sequence; Ampr | IBA |
| pTrc99a | Vector for IPTG-inducible gene expression; Ampr | 42 |
| pET16b | Vector for IPTG-inducible gene expression; carries an N-terminal His10 tag sequence; Ampr | Novagen |
| pBAD24 | Vector for arabinose-inducible gene expression; Ampr | 43 |
| pBS2E | Empty vector for integration at amyE; Ampr; mlsr | 44 |
| pKT25 | Vector for translational fusions of cyaA T25 fragment to N terminus of insert polypeptide; lac promoter; Kanr | 32 |
| pKTN25 | Vector for translational fusions of cyaA T25 fragment to C terminus of insert polypeptide; lac promoter; Kanr | 32 |
| pUT18 | Vector for translational fusions of cyaA T18 fragment to C terminus of insert polypeptide; lac promoter; Ampr | 32 |
| pUT18C | Vector for translational fusions of cyaA T18 fragment to N terminus of insert polypeptide; lac promoter; Ampr | 32 |
| pSDIBA501 | pASK-IBA5C-bceAB | This study |
| pSD2402 | pBAD24-bceAB-Strep | This study |
| pRU2401 | pTrc99a-bceS-His8 | This study |
| pCF120 | pET16b-bceR-His10 | This study |
| pNT2E07 | pBS2E-Pxyl-bceS | This study |
| pNT2E01 | pBS2E-Pxyl-bceAB | This study |
| pSD2E01 | pBS2E-Pxyl-bceS-His8 | This study |
| pSD2E02 | pBS2E-Pxyl-Strep-bceAB | This study |
| pAS1803 | pUT18-bceS | 33 |
| pAS2503 | pKT25-bceS | This study |
| pCF18C01 | pUT18C-bceR | This study |
| pCF2501 | pKT25-bceR | This study |
| pCF18C02 | pUT18C-bceRS | This study |
| AS1804 | pUT18-bceA | 33 |
| pAS2504 | pKT25-bceA | This study |
| pAS1805 | pUT18-bceB | This study |
| pAS2505 | pKT25-bceB | 33 |
| pHF1804 | pUT18-bceAB | This study |
| pHF2509 | pKT25-bceAB | This study |
| E. coli strains | ||
| XL1-blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 Tetr] | Stratagene |
| DH5α | fhuA2 lac (Δ)U169 phoA glnV44 Φ80′ lacZ (Δ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | 45 |
| BTH101 | F−, cya-99, araD139, galE15, galK16, rpsL1, hadR2, mcrA1, mcrB1 | Euromedex |
| C43(DE3) | F− ompT gal dcm hsdSB (rB− mB−) (DE3) | 46 |
| SGE217 | C43 (DE3); arnC::kan | This study |
| B. subtilis strains | ||
| TMB1557 | ΔbceS; sacA::PbceA-luxABCDE | This study |
| SGB79 | bceAB::kan; sacA::PbceA-luxABCDE | This study |
| SGB276 | ΔbceS; sacA::PbceA-luxABCDE; lacA::Pxyl-bceS-His8 | This study |
| SGB277 | bceAB::kan; sacA::PbceA-luxABCDE; lacA::Pxyl-Strep-bceAB | This study |
| TMB1650 | ΔbceS; sacA::PbceA-luxABCDE; lacA::Pxyl-BceS | This study |
| TMB1636 | bceAB::kan; sacA::PbceA-luxABCDE; lacA::Pxyl-bceAB | This study |
| SGB276 | ΔbceS; sacA::PbceA-luxABCDE; lacA::Pxyl-BceS-His8 | This study |
| SGB277 | bceAB::kan; sacA::PbceA-luxABCDE; lacA::Pxyl-Strep-bceAB | This study |
a The abbreviations used are as follows: Ampr, ampicillin resistance; kanr, kanamycin resistance; mlsr, macrolide-lincosamide-streptogramin B resistance.