TABLE 2.
Primers used in this study
| Name | Sequence (5̍′ to 3̍′)a | Used for amplification of |
|---|---|---|
| 2256 | AATTGGTCTCAGCGCCATGGTGATTTTAGAAGCG | Strep-bceAB (fwd) |
| 2257 | AATTGGTCTCGTATCACAACGACGATTTAATG | Strep-bceAB (rev) |
| 2007 | ATCGCTCGAGTTGTTTAAACTTTTGCTGATTG | bceR-His10 (fwd) |
| 2008 | ATCGGGATCCTTAATCATAGAACTTGTCCTC | bceR-His10 (rev) |
| 1895 | AATTCCATGGTTAAAGCATTCCTTATCGAAAGG | bceS-His8 (fwd) |
| 1905 | AATTCTGCAGTCAGTGATGGTGATGGTGATGGTGATGCACGCTTATGACATGTTCAAATTG | bceS-His8 (rev) |
| 1525 | AATTCCATGGTGATTTTAGAAGCGAA | bceAB-Strep (fwd) |
| 2201 | AATTGTCGACTTATTTTTCGAACTGCGGGTGGCTCCAGCCACCGCCACCGCCACCCAACGACGATTTAATGACC | bceAB-Strep (rev) |
| 1355 | GTCATCTAGAGATGATTAAAGCATTCCTTATCG | bceS (fwd) BACTH |
| 1356 | GTCAGGTACCTGCACGCTTATGACATGTTC | bceS (rev) BACTH |
| 1359 | GTCATCTAGAGATGAACATTAATCAGCTCATCC | bceB (fwd) BACTH |
| 1360 | GTCAGGTACCTGCAACGACGATTTAATGACC | bceB (rev) BACTH |
| 1357 | GTCATCTAGAGATGGTGATTTTAGAAGCG | bceA (fwd) BACTH |
| 1358 | GTCAGGTACCTGATGTTCATGCTGCACC | bceA (rev) BACTH |
| 3218 | AATTTCTAGAGTTGTTTAAACTTTTGCTGATTGAAG | bceR (fwd) BACTH |
| 3219 | AATTGGTACCTGTTAATCATAGAACTTGTCCTCTTC | bceR (rev) BACTH |
a Restriction sites are shown in bold; nucleotides encoding the His8 tag are shown underlined; nucleotides encoding the Strep-tag are shown in italics; fwd, forward; rev, reverse.