FIGURE 10.
Equilibrium binding of SK and SKΔK414 to [5F]FFR-[Lys]Pg in the presence of benzamidine. The fractional change in fluorescence (ΔF/Fo) of 20 nm [5F]FFR-[Lys]Pg in buffer containing 50 mm benzamidine is plotted as a function of the total nSK (●) or SKΔK414 (○) concentration ([SK]o or [SKΔK414]o). Solid lines represent least square fits of the quadratic equation for binding of a single ligand with the parameters listed in the text and Table 1. Fluorescence titrations were performed and analyzed as described under “Experimental Procedures.”