FIGURE 2.

SK concentration dependence of the kinetics of [5F]FFR-[Lys]Pg binding in the absence of lysine analogs. Dependences of k_{obs 1} and k_{obs 2} (●, nSK, fluorescence intensity; ○, nSK, fluorescence anisotropy; ▴, WT-SK, fluorescence intensity) on [SK]_o are shown for binding to 10–20 nm [5F]FFR-[Lys]Pg. The inset shows the k_{obs 2} dependence on an enlarged scale. Solid and dashed lines represent the least square fits by Equation 2 with the parameters given in Table 1 for the reactions with nSK and WT-SK, respectively.