FIGURE 4.

HDAC3 regulates the invasion, migration, and the growth potential of cancer cells. A, cellular proliferation of was determined by trypan blue exclusion assay. Each value represents an average of three independent experiments. B, indicated cancer cell line was harvested, counted, resuspended in 0.2% soft agar, and seeded onto 0.4% soft agar supplemented with 10% fetal bovine serum (1000 cells/well for Malme3M/Malme3M^R cells). Four weeks later, colonies were stained and counted. The columns represent mean of triplicate experiments; bars indicate S.D.; *, p < 0.05. C, indicated cancer cell line was subjected to invasion assays. Malme3M^R-HDAC3-FLAG denotes cells that stably express HDAC3-FLAG. Malme3M ^AS-HDAC3 denotes cells that stably express antisense HDAC3. **, p < 0.005; ***, p < 0.0005. D, Malme3M or Malme3M^R cells were transfected with the construct as indicated, followed by anchorage-independent growth assays. Each value represents average of three independent experiments. E, indicated cancer cell lines were subjected to wound migration assays. Movement of cells into wound was shown for the indicated cancer cell lines at 0 and 48 h post-scratch (×40). The broken lines indicate the boundary lines of scratch. F, data were the means of three independent experiments, and the bars represent S.D. of the mean. G, cell lysates were subjected to Western blot analysis. ns denotes not significant.