FIGURE 5.

HDAC3 confers sensitivity to microtubule-targeting drugs in vivo. A, Malme3M^R-Vector (1 × 10⁶) or Malme3M^R-HDAC3-FLAG cells (1 × 10⁶) were injected into the dorsal flanks of athymic nude mice. Taxol (1 mg/kg) was injected into each nude mouse after the tumor reached a certain size. Tumor volume was measured as described. Each experimental group consisted of five mice. Each value represents an average obtained from the five athymic nude mice of each group. Data are expressed as mean ± S.D. Tumor lysates were subjected to Western blot analysis (right panel). B, Malme3M^Vector (1 × 10⁶), Malme3M^R-vector (1 × 10⁶), or Malme3M^R-HDAC3-FLAG cells (1 × 10⁶) were injected into the dorsal flanks of athymic nude mice. Celastrol (1 mg/kg) or vinblastine (0.5 mg/kg) was injected into each nude mouse after the tumor reached a certain size. Tumor volume was measured as described. Each experimental group consisted of five mice. Each value represents an average obtained from the five athymic nude mice of each group. Data are expressed as mean ± S.D. Each figure shows a representative image of the mice in each group at the time of sacrifice. Tumor-bearing mice were assessed for weight loss. All animal experiments were approved by Institutional Animal Care and Use Committee (IACUC) of Kangwon National University (KIACUC-13-0005). Lysates isolated from each tumor tissue were subjected to Western blot analyses. C, Malme3M^Vector (1 × 10⁶) or Malme3M^AS-HDAC3 cells (1 × 10⁶) were injected into the dorsal flanks of athymic nude mice. Celastrol (1 mg/kg) or Taxol (1 mg/kg) was injected into each nude mouse after the tumor reached a certain size. Tumor volume was measured as described. Each experimental group consists of five mice. Each value represents an average obtained from five athymic nude mice of each group. Data are expressed as mean ± S.D. Tumor lysates were also subjected to Western blot (lower panel).