FIGURE 7.

p53 interacts with HDAC3 and is responsible for the binding of HDAC3 to the promoter sequences of miRNAs. A, cell lysates were immunoprecipitated with the indicated antibody (2 μg/ml), followed by Western blot analysis or subjected to Western blot analysis (left panel). The indicated cancer cell line was transfected with control vector (1 μg/ml) or HDAC3-FLAG (1 μg/ml). 48 h after transfection, Western blot analysis was performed (right panel). B, Malme3M cells were transiently transfected with the indicated siRNA (10 nm). 48 h after transfection, Western blot analysis was performed. C, same as B except that ChIP assays were performed. IP, immunoprecipitation.