FIGURE 9.

Inhibition of miR-200b, miR-217, or miR-335 confers resistance to anti-cancer drugs and enhances the migration and invasion potential of Malme3M^R-miR-200b, Malme3M ^R-miR-217, or Malme3M^R-miR-335 cells. A, indicated cancer cells were transiently transfected with the indicated inhibitor (50 nm each). 48 h after transfection, Western blot analysis was performed. B, same as A except that qRT-PCR analysis was performed. C, indicated cancer cells transiently transfected with the indicated inhibitor (50 nm each) were subjected to wound migration assays. D, same as C except that invasion assays were performed. E, Malme3M cells were transiently transfected with the indicated inhibitor (50 nm each). 24 h after transfection, cells were treated with Taxol (1 μm) for 24 h, followed by MTT assays. F, same as E except that caspase-3 activity assays were performed. G, same as F except that Western blot analysis was performed. Ctrl. Inh., control inhibitor.