Figure 1.

Effect of isoflurane exposure on expression and translocation of HIF-1α in cultured human prostate cancer cells (PC3 cells). Cultured cells were exposed to 0.5–2% for 2 h and were harvested immediately or 2–24 h after exposure for western blotting. (A, B) A time- and concentration-dependent increase in HIF-1α expression after isoflurane exposure; (C) immunostaining further revealed isoflurane exposure promoted the translocation of HIF-1α from cytoplasm to nucleus where its downstream effects are initiated; (D, E) HIF-1β expression was unchanged after exposure to isoflurane. Results are expressed as mean±s.d.; *P<0.05; **P<0.001 vs naïve control. Bar=25 μm.