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. 2014 Aug 5;111(7):1400–1409. doi: 10.1038/bjc.2014.435

Figure 1.

Figure 1

Insulin-like growth factor binding protein-2 overexpression in glioma cells enhances invasion but not proliferation. (A) Insulin-like growth factor binding protein-2 expression in four glioblastoma cell lines and primary SU3 glioma cells, as determined by RT–PCR and western blot analysis. (B) pEGFP-N1-IGFBP-2 plasmid or control pEGFP-N1 was effectively transfected into U87 cells. (C) Insulin-like growth factor binding protein-2 overexpression did not significantly affect cell proliferation as shown by MTT assay and cell counting. (D) Invasion assay showed that compared with cells transfected with control vector and untransfected cells, the invasive ability of U87-pEGFP-N1-IGFBP-2 cells was markedly increased. (E) The phosphorylation of ERK was not markedly altered by the levels of IGFBP-2 expression. (F) Inhibition of ERK did not affect the invasion induced by IGFBP-2 overexpression.