Table 1.
Primer Sequences and Restriction Endonucleases for Three SNPs
| SNPs | Base change | Allele frequencies in HapMap CHB | Primersa | Enzymes | Digested fragments (bp) |
|---|---|---|---|---|---|
| rs3916840 | C/T | C 0.942/T 0.058 | F: GTCTGTCTCCTACTGGACTGCGTA | Csp6I | C (21,198) |
| R: ATAAGTTCTGGGGGGTTAGGGATG | T (219) | ||||
| rs1799793 | G/A | G 0.933/A 0.067 | F: ACCTGGCCAACCCCGTGCTGCTC | TaqI | G (22,199) |
| R: TCTCCTGCACCACATGCTGCACAC | A (221) | ||||
| rs238416 | G/A | G 0.512/A 0.488 | F: GGGGGTCAAGGTTCATTTTTTGGTTCCATCCG | MspI | G (30,182) |
| R: TGACTGTGAGGCAGGCAGAGCCAATCAGAG | A (212) |
a
Sequences for primers and probes in 5′ to 3′ order.
CHB, Han Chinese in Beijing, China; F, forward; R, reverse; SNP, single nucleotide polymorphism.