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. 2014 May 21;155(8):3054–3064. doi: 10.1210/en.2013-2116

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Copyright © 2014 by the Endocrine Society

This article has been published under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright for this article is retained by the author(s). Author(s) grant(s) The Endocrine Society the exclusive right to publish the article and identify itself as the original publisher.

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Figure 1. — Temporal mRNA expression of type I, type II, and accessory receptors specific for the ligand BMP4 in chorionic girdle tissue from day 27, 30, 31, and 34 conceptuses. A, Qualitative RT-PCR analysis of temporal receptor expression in the chorionic girdle. Amplicons were generated using primers specific for equine BMPR1A, BMPR1B, BMPR2, BAMBI, DRAGON, GCM1, and BACTIN (as control) mRNA, and all bands observed in all tissues with each primer set are of the correct predicted size: 306, 343, 364, 264, 269, 450, and 346 bp, respectively. The figure shows typical RT-PCR product gels (n = 3) in which the tissue-specific mRNA expression profile was representative of 3 independent chorionic girdles. NTC, no template control. Equine testis and nonpregnant uterus were used as control tissues (lanes marked +VE). B, Real time quantitative RT-PCR expression of BMPR1A, BMPR2, DRAGON, and BAMBI mRNA in days 27–34 chorionic girdle. Data points represent the values for individual conceptuses (n = 4, except for day 27 [n = 3] because one sample failed to meet control gene validation parameters and was excluded from the dataset).