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Subcellular distribution of GLUT4 in skeletal muscle fibers, as revealed by immunogold electron microscopy. Insulin (5 IU/kg body wt) was administered intravenously to rac1flox/flox and m-rac1-KO mice (8–10 wk old), and subcellular distribution of GLUT4 in gastrocnemius muscle fibers was analyzed by immunogold electron microscopic analysis. Arrows indicate GLUT4 labeling in sarcolemma. G, Golgi apparatus; M, mitochondrion; S, sarcolemma; SR, sarcoplasmic reticulum. Scale bar = 500 nm.
