Skip to main content
. 2014 Sep 19;4(12):1193–1208. doi: 10.7150/thno.8712

Figure 1.

Figure 1

Expression, functions and the targets of miR-194 in CRC cells. (A) Real-time PCR quantification of miR-194 in seven human CRC cell lines. (B) CCK-8 assay was performed in HCT116 and SW1116 cells transfected with miR-194 mimics or control miRNA. Cell viability was measured from absorbance reading at 450nm. Data was expressed as relative viability (%) calculated: [A450(treated)-A450(blank)]. (C) In vitro invasion and migration assays were performed using HCT116 and SW1116 cells. Cells were photographed under a light microscope and counted from five random microscopic fields (200×) per insert in triplicate. (D) mRNA levels of PDK1, AKT2 and XIAP after miR-194 mimics transfection in HCT116 and SW1116 cells. (E) Schematic illustration of the predicted miR-194-binding sites in PDK1, AKT2 and XIAP 3'-UTR. MiR-194 mimics and report constructs, containing a wild-type or a mutated PDK1, AKT2 or XIAP 3'-UTR, were co-transfected into HCT116 cells respectively. Relative repression of firefly luciferase expression was standardized to a transfection control. n=3, ANOVA. All experiments were repeated at least three separate times, Data are shown as mean ± SD, *P<0.05, **P<0.01(Student's t-test), compared with the control miRNA.