Figure 2.

Growth dynamics of C. albicans (squares) and P. aeruginose (circles) in (a) cocultures and (b) monocultures. Growth was based on colony-forming units (CFU). (c, d) Epifluorescence images from mixed C. albicans–P. aeruginosa biofilms stained with the LIVE/DEAD staining system are shown. A viability assay confirmed that C. albicans cells lost viability after 48 h in mixed biofilms. Red cells (stained with propidium iodide) are considered dead, while live cells remained green (stained with SYTO9). The panels present the means±s.e.m. (n=3).