. Author manuscript; available in PMC: 2014 Oct 3.

Published in final edited form as: J Surg Res. 2013 Jan 1;184(2):730–738. doi: 10.1016/j.jss.2012.12.009

Table 1. Rules for Pseudomonas aeruginosa (PA) and epithelial cell agent behavior.

The overall logic of the model is based on the behaviors of each individual agent type. Each agent executes the outlined rules, which manifest as specific behaviors within the model space at both the individual agent and population levels. The epithelial cell agent behaviors are described in considerably more detail in Stern, et al¹⁹

BEHAVIOR	PA AGENT RULE/ACTION
Movement	Random
Activation	If [ROS] > threshold → activate
Binding	If activated and appropriate receptor available, stop moving and attach (Integrin α3β1, LM-332, LM-511)
Virulence Expression	If activated, produce virulence factors P2-SEC → Secrete toxin into environment P2-INJ → Inject toxin into epi cell when bound

BEHAVIOR	EPITHELIALAGENT RULE/ACTION
Sensing stimulatory signals (DAMPs, ROS, ATP)	Ligand-receptor binding → signal transduction to effector molecules for migration, spreading
Healing	Coordinated cell migration, spreading
Tight junction disruption	Wound-, radiation- or toxin-mediated; exposes basolateral receptors
Death	When [toxin] > threshold → die (extracellular or intracellular)