Fig. 7.

Sample normalization. A Levy-Jenning plot showing iterative analysis over a 2-year period of aliquots from a single sample (CHI-002) stained with our 15-color T regulatory cell tube. a T cells and T cell subpopulations CD4⁺CD8⁻, CD4⁺CD8⁺, CD4⁻CD8⁺, CD4⁻CD8⁻ are indicated. b Discrete markers such as activation markers on T CD4⁺ cells and T CD8⁺ cells and on CD4⁺ regulatory T cells were identified