Table 1.
Instrument configuration
| Laser wavelength (nm) | Laser color | Laser power (mW) | Laser type | Spectral range for detection (nm) | Dichroic #1 (nm) | Dichroic #2 (nm) | Band Pass (nm) | Fluorochrome |
|---|---|---|---|---|---|---|---|---|
| 639 | red | 40 | cube | 750–810 | 740LP | 780/60 | APC Cy7/APC H7 | |
| 685–735 | 740LP | 685LP | 710/50 | AF700 | ||||
| 630–670 | 685LP | 660/20 | APC/AF647 | |||||
| 532 | green | 150 | compass | 760–800 | 740LP | 780/40 | PE-Cy7 | |
| 685–735 | 740LP | 690LP | 710/50 | PE-Cy5.5 | ||||
| 640–680 | 690LP | 640LP | 660/40 | PE-Cy5 | ||||
| 600–620 | 640LP | 600LP | 610/20 | PE-TR | ||||
| 562–588 | 600LP | 575/25 | PE | |||||
| 488 | blue | 50 | saphire LP | 685–735 | 685LP | 685LP | 710/50 | PerCP Cy5.5 |
| 505–525 | 505LP | 505LP | 515/20 | FITC/AF488 | ||||
| 406 | violet | 100 | cube | 750–810 | 740LP | 780/60 | QD800 | |
| 670–740 | 740LP | 670LP | 705/70 | x | ||||
| 640–680 | 670LP | 630LP | 660/40 | QD655 | ||||
| 585–625 | 630LP | 595LP | 605/40 | QD605 | ||||
| 570–595 | 595LP | 570LP | 585/42 | x | ||||
| 540–580 | 570LP | 557LP | 560/40 | x | ||||
| 505–535 | 557LP | 505LP | 515/20 | Aqua blue | ||||
| 425–475 | 450/50 | V450 | ||||||
| 355 | ultraviolet | 60 | genesis | 500–575 | 505LP | 505LP | 525/50 | UV525 |
| 425–475 | 450/50 | UV450 |
DPSS Diode Pumped Solid State
22 PMT are in this instrument, but only 20 are useable thanks to a switch between 2 PMT on violet and ultraviolet laser