. Author manuscript; available in PMC: 2015 Jan 1.

Published in final edited form as: Curr Top Microbiol Immunol. 2014;377:23–60. doi: 10.1007/82_2013_336

Table 2.

CLIP panel

CHI CLIP panel
			Lymphoid
			T lineage			B lineage			NK lineage
Excitation	Fluorochrome name	PMT name	T₁	T₂	T₃	B₁	B₂	B₃	NK₁	NK₂
407 nm Excitation	V450	V450	CD4	CD4	CD4	Lambda	CD80	CD80	CD56	CD56
				RPA-T4	RPA-T4	RPA-T4	B39-4	L307.4	L307.4	B159
	Aquablue	V545	Viability	Viability	Viability	Viability	Viability	Viability	Viability	Viability
	Qdot 605	V605	CD8	CD8	CD8	CD10	CD27	CD27	CD8	CD8
			3B5	3B5	3B5	MEM-78	CLB-27/1	CLB-27/1	3B5	3B5
	Qdot 655	V655	CD27	CD27	CD27	CD19	CD19	CD19	CD30	NKp46
			CLB-27/1	CLB-27/1	CLB-27/1	SJ25-C1	SJ25-C1	SJ25-C1	BerH8	900
	Qdot 800	V800	CD45	CD45	CD45	CD45	CD45	CD45	CD45	CD45
			HI30	HI30	HI30	HI30	HI30	HI30	HI30	HI30
488 nm Excitation	FITC/Alexa488	B515	CD39	IL-23R	CD69	CD40	IgA	IgA (intra)	CD57	CD57
			A1	218213	FN50	5C3	Polyclonal Goat	Polyclonal Goat	TB01	TB01
	PcPcy5.5	B710	CD38	CD196	IL-4	CD138	CD86	CD86	CD161	IFNg
			HIT2	11A9	MP4-25D2	MI15	IT2.2	IT2.2	HP-3G10	4S.B3
532 nm Excitation	PE	G560	Foxp3	CD146	perforin	Kappa	CD21	IgE (intra)	CD337	Perforin
			PCH101	142928	δG9	TB28-2	BL13	MB10-5C4	P30-15	δG9
	PE-TR	G610	CD45-RA	CD45-RO	CD45-RO	CD38	CD38	CD38	CD94	CD25
			2H4LDH11LDB9	UCHL1	UCHL1	HIT2	HIT2	HIT2	DX22	B1.49.9
	PE-cy5	G660	CD103	CD161	CD40L	CD103	IgG	IgG (intra)	CD16	CD16
			LF61	DX12	TRAP1	LF61	G18-145	G18-145	3G8	3G8
	PE-cy5.5/PE-A700	G710	HLA-DR	TNFa	TNFa	CD20	CD20	CD20	CD244	CD69
			Tu36	MP9-20A4	MP9-20A4	HI47	HI47	HI47	C1.7	CH/4
	PE-cy7	G780	CD25	IL-17A	IFNg	CD5	CD23	CD23	CD5	CD5
			M-A251	BL168	4S.B3	L17F12	EBVCS-5	EBVCS-5	L17F12	L17F12
633 nm Excitation	APC/Alexa647	R660	CD127	IL-22	IL-2	CD22	IgD	IgD (intra)	CD336	CD127
				hIL-7R-M21	142928	MQ1-17H12	S-HCL-1	IgD26	IgD26	P44-8
	Alexa700/APC-cy5.5	R710	CD197	CD197	CD197	CD11c	IgM	IgM (intra)	CD158e1	CD158e1
			150503	150503	150503	3.9	CH2	CH2	DX9	DX9
	APC-cy7/APC-H7	R780	CD3	CD3	CD3	CD25	CD10	CD10	CD3	CD3
			Sk7	Sk7	Sk7	M-A251	HI10a	HI10a	Sk7	Sk7

			Myeloid
			Dendritic lineage	Mono lineage		Neutrophil lineage
Excitation	Fluorochrome name	PMT name	DC₁	M₁	M₂	N₁	N₂
407 Excitation	V450	V450	CD123	HLA-DR	CD31	CD62L	CD14
			6H6	L243 (G46-6)	WM59	DREG-56	TuK4
	Aquablue	V545	Viability	Viability	Viability	Viability	Viability
	Qdot 605	V605	CD27	CD163	CD163	CD3	CD8
			CLB-27/1	eBioGHI/61	eBioGHI/61	sk7	3B5
	Qdot 655	V655	CD4	CD14	CD14	CD14	CD19
			S3.5	TuK4	TuK4	TuK4	SJ25-C1
	Qdot 800	V800	CD45	CD45	CD45	CD16	CD45
			HI30	HI30	HI30	3G8	HI30
488 Excitation	FITC/Alexa488	B515	IFNa	CD77	CD77	CD185	CD123
			MMHA-11	5B5	GA-R2 (HIR2)	2G8
	PcPcy5.5	B710	TNFa	CD192	CD34	CD203c	CD4
			MAb11	TG5/CCR2	8G12	NP4D6	S3.5
532 nm Excitation	PE	G560	CD40	CD64	CD64	CD303	CD56
			HB14	10.1	10.1	201A	NCAM16.2
	PE-TR	G610	HLA-DR	CD16	CD16	CD123	CD16
			Tu36	3G8	3G8	6H6	3G8
	PE-cy5	G660	CD83	CD15	CD15	CD15	CD15
			HB15e	80H5	80H5	80H5	80H5
	PE-cy5.5 /PE-A700	G710	CD11c	CD4	CD4	HLA-DR	HLA-DR
			Bu15	S3.5	S3.5	Tu36	Tu36
	PE-cy7	G780	lineage	CD13	CD13	CD13	CD34
			see below	L138	L138	L138
633 nm Excitation	APC/Alexa647	R660	BDCA-2	CD36	CD36	FcεRI	CD11c
			AC144	CB38 (NL07)	CB38 (NL07)	BC4	Bu15
	Alexa700/APC-cy5.5	R710	CD33	CD33	CD33	CD33	CD33
			WM-53	WM-53	WM-53	WM-53	WM-53
	APC-cy7/APC-H7	R780	CD86	CD206	HLA-DR	CD10	CD3
			IT2.2	15-2	L243 (G46-6)	HI10a	Sk7

Lineage mix =

CD3 SK7

CD56 NCAM16.2

CD20 L27

CD19 SJ25C1

CD14 M5E2

CD16 3G8