Fig. 1.

CRISPR-GA pipeline. (A) From experiment to report. Schematic pipeline of gene editing assessment. (B) Output of CRISPR-GA: different information is estimated. Deletions, insertions, HR and corresponding efficiencies. Upper panels estimate the number of insertions and deletions and each corresponding size. Middle panels estimate the number of insertions and deletions, and their corresponding location within the genomic locus of interest. The bottom panel shows the number of deletions and HR at each corresponding locations and outputs the HR and NHEJ efficiency. (C) Experimental results assessed by CRISPR-GA from testing several mutants of cas9, gRNAs and a DNA template. HR and NHEJ values are presented